Ni-IDA Resin

Description:

High-Affinity Ni-IDA Resin (Cat. No. RG00223, 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (iminodiacetic acid, called IDA) that binds Ni²⁺ ions at three coordination sites, It is particularly useful for the high-affinity purification of 6xHis-tagged recombinant proteins from any recombinant expression system, including bacterial, yeast, insect cell, and mammalian cells. These His-tagged recombinant proteins can be purified under either native or denaturing conditions. Proteins bound to the resin are then eluted with either low-pH buffer or imidazole solution or even with histidine solution.

Key Features:

• High binding capacity: The kit can handle over 10 mg 6xHis-tagged protein/ml (CV).
• Simple purification procedure under both native and denaturing conditions.
• Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH14 for short periods.
• Resilient: If properly regenerated, the resin can be reused multiple times.

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

Storage:

Store the product between 4°C and 8°C.

Note:

• For ambient pressure use only.